›› 2009, Vol. 36 ›› Issue (9): 101-103.

• 遗传繁育 • Previous Articles     Next Articles

Effects of Cytochalasin B on Vitrification of Bovine GV-stage Oocytes

GUO Juan1,2, WANG Zhi-qin1, ZHAO Yun-cheng2, LIN Jia-peng1, HUANG Jun-cheng2   

  1. (1.Department of Veterinary Medicine,Xinjiang Agriculture University, Urumqi 830052,China; 2.The Key Laboratoy of Animal Biotechnology of Ministry of Agriculture, Xinjiang Academy of Animal Sciences,Urumqi 830000,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: HUANG Jun-cheng

Abstract: In the present study, effects of concentration of cytochalasin B(CB) on solid surface vitrification (SSV) of bovine GV-stage oocytes were examined. The results demonstrated that no significant differences were observed in vitro maturation rate among with or without CB pre-treatment,while they were all significantly lower than that in the IVM group.In conclusion,different concentration CB(2.5,7.5,15,20 and 30 μg/mL)treatment before vitrification did not improve the IVM rate following the vitrification of immature bovine oocytes.

Key words: vitrification; cytochalasin B; bovine; GV-stage oocytes

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