猪戊型肝炎病毒ORF2部分片段原核表达载体的构建

›› 2011, Vol. 38 ›› Issue (10): 214-217.

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The Construction of Prokaryotic Expression Vector of Swine Hepatitis E Virus ORF2 Fragment

HAO Bao-cheng^1,2, LAN Xi², HU Yong-hao³, LIU Ji-xing^2,3, LIANG Jian-ping^1,3

1. Key Laboratory of New Animal Drug Project,Gansu Province/Key Laboratory of Veterinary Drug Discovery,Agricultural Ministry/Lanzhou Institute of Husbandry and Pharmaceutical, Chinese Academy of Agricultural Sciences, Lanzhou 730050,China;2. Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences, Lanzhou 730046,China;3. College of Veterinary,Medical Gansu Agricultural University,Lanzhou 730070,China

Received:2011-03-01 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

Abstract

Abstract: The test used the Protean software to analyze the potential antigenic sites of ORF2 of HEV swCH189 strain,and selected 381aa to 623aa segment as the expression of target,the cp239 fragment was amplified from the full-length ORF2 plasmid by PCR,after purification,connection,and so on,the prokaryotic expression vector pET32a(+)-cp239 was successfully constructed. This work laid the foundation for the next step of the prokaryotic expression and analysis of biological activity.

Key words: swine; hepatitis E virus; ORF2; construction of prokaryotic expression vector

CLC Number:

HAO Bao-cheng;LAN Xi;HU Yong-hao;LIU Ji-xing;LIANG Jian-ping;. The Construction of Prokaryotic Expression Vector of Swine Hepatitis E Virus ORF2 Fragment[J]. , 2011, 38(10): 214-217.

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The Construction of Prokaryotic Expression Vector of Swine Hepatitis E Virus ORF2 Fragment

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