China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (10): 117-121.

• 遗传繁育 • Previous Articles     Next Articles

Genetic Polymorphism of the Exon 2 of MHC-DQB Gene in Yunnan Humped Cattle

YU Wen-hai1, LU Shao-xiong2, HE Zhan-long1, LIU Hong-wen3, LU Run-feng4, JIN Jian-bin4   

  1. 1. Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;3. Domestic Animal Improving Station of Yunnan Province, Kunming 650224, China;4. Bureau of Animal Husbandry, Guangnan County of Yunnan Province, Guangnan 663300, China
  • Received:2011-03-20 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

Abstract: In this paper, the genetic polymorphism of the exon 2 of MHC-DQB gene was investigated by directly sequencing and PCR-RFLP in Yunnan humped cattle. Homology of the nucleotide acid sequences was analysis by DNAMAN software. The results showed that 17 alleles were found in this experiment,10 kinds of genotypes and 5 (A, B, C, D, E) alleles were found with enzyme Hae Ⅲ;22 kinds of genotypes and 11 (A, B, C, D, E, F, G, H, I, J, K) alleles were found with enzyme RsaⅠ;only one kind of genotype were found with enzyme TaqⅠ. Polymorphic sites were detected at base position 12, 25, 63, 96, 106, 126, 152, 156, 165 and 204. The results showed that there were same lengths (270 bp) of gene fragment in various species, but there was a nucleotide missing at position 224 and a nucleotide insertion at position 236 in Yunnan humped cattle. The homology between Yunnan humped cattle and human, swine, horse, sheep, Yellow cattle × Zebuand were 81.4%, 83.3%, 78.1%, 87.7% and 86.6%, respectively. The results of χ2 test showed that the polymorphic loci of Hae Ⅲ and TaqⅠwere in Hardy-Weinberg equilibrium (P＞0.05), but the locus of RsaⅠ was not in equilibrium (P＜0.05).

Key words: Yunnan humped cattle; MHC; DQB gene; exon 2; genetic polymorphism

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