Abstract: The methods of mice ear-swelling and granulation tissue hyperplasia of mice induced by tampon were adopted to study the anti-inflammatory action of pure traditional Chinese herbal medicine for dairy cow mastitis,and improved Karbr was adopted to study the toxic effect of the Chinese herbal medicine prescription with LD₅₀ as the evaluate index of the acute toxicity strength. The test results indicated that the Chinese herbal medicine prescription to mice ear swell were 10.41,9.26,8.82 mg,respectively in the three test groups(high dosage,middle dosage and low dosage of the prescription). There had significant difference between the control group(12.52 mg) and test groups(P<0.05). Mice granulation tissue hyperplasia in the Chinese herbal medicine prescription groups were 11.21,10.71,8.83 mg,respectively. There had significant difference between the control group (12.63 mg) and test groups(P<0.05). In the actute toxicity test,none mouse was dead,so the LD₅₀ of the prescription were more than 60000 mg/kg·bw. The results showed that the anti-inflammation action of the Chinese herbal medicine prescription was significantly effective for both of active and chronic inflammation period. The Chinese herbal medicine prescription belongs to a practical innoxious substance and can be used in the clinical research.

Key words: Chinese herbal medicine; mice; anti-inflammatory test; acute toxicity test