Abstract: Buffalo is susceptible animals to Fasciola gigantic. To clarify the mechanism of immune,mononuclear macrophage cells have a very important role. In this experiment, buffalo peripheral blood mononuclear cells were separated by centrifugation, cultured in plates and then got adherent monouclear cells. The number of the adherent monouclear cells in culturein vitro. Adherent cells cultured 72 h identificate by inverted microscope.Wright’s staining indicated that those cells had the cell morphology of mononuclear macrophage cell.Acid phosphatase (positive rate 72.8%±0.5%), non-specific esterase (positive rates 84.6%±0.4%) had the cell cytochemical characteristics. The immunocytochemistry for the the adherent cells found that they display typical functional membrane protein MHC Ⅱ. Phagocytosis (positive rates 87.5%±0.6%)was measured by Chinese ink.The result indicated the adherent cells retain the phagocytic activities. The results showed that adherent cells from buffalo blood culture in 72 h have mononuclear macrophage characteristics and could used to the future study at culture in 72,96,120 h in vitro.

Key words: mononuclear macrophage; Fasciola gigantic; water buffalo; cell culture; identification