›› 2012, Vol. 39 ›› Issue (3): 125-129.

• 生理生化 • Previous Articles     Next Articles

Screening,Identification of a Strain with High Keratinase Production and the Primary Study of its Characterization of Fermentation

GUO Gang1, CHU Jie1,2, WANG Jun-gao1, LIU Ke-chun2   

  1. 1. School of Food & Bioengineering, Shandong Institute of Light Industry, Jinan 250353, China;2. Biology Research Institute of Shandong Academy of Science, Jinan 250014, China
  • Received:2011-11-09 Revised:1900-01-01 Online:2012-03-20 Published:2012-03-20

Abstract: A strain with high keratinolytic activity was obatained by casein plate firstly and feather as the sole carbon source and nitrogen source in submerged medium secondly.The strain S6 was identified as Bacillus licheniformis based on its morphologic and physicochemical characteristics.After fermenting 72 h in submerged culture,the Bacillus licheniformis S6 demonstrated highest keratinolytic activity and maximum of soluble protein, 186.8 U/mLand 160.87 μg/mL,respectively,which expresses that Bacillus subtilis S6 has a promising prospect in the degradation process of feather.

Key words: screen; identification; Bacillus licheniformis; keratinolytic activity; soluble protein

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