猪繁殖与呼吸综合征病毒NVDC-JXA1株膜基质蛋白单克隆抗体的研制

China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (11): 70-73.

• 生物技术 • Previous Articles Next Articles

Development of Monoclonal Antibodies against Matrix Protein ofPRRSV NVDC-JXA1 Strain

WANG Yuan^1，2， HU Hong-hui^2，3， QIN Ya-man²， ZHANG Qian²， DENG Xiao-yu²，CAO Zhen²， WANG Chuan-bin²， RAN Duo-liang¹， TIAN Ke-gong^2

（1.College of Veterinary Medicine，Xinjiang Agricultural University，Urumqi 830052，China；2.China Animal Disease Control Center，Beijing 100193，China；3.College of Veterinary Medicine，China Agricultural University，Beijing 100193）

Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
Contact: CAO Zhen

Abstract

Abstract: To develop and characterize monoclonal antibody (McAb) against porcine reproductive and respiratory syndrome virus NVDC-JXA1 strain (PRRSV NVDC-JXA1 strain) for related research work.BALB/c mice were immunized conventionally with the purified virions of PRRSV NVDC-JXA1 strain.On the third day after the final immunization，spleen cells of mice fused with myeloma cells SP2/0 were filtered by culturing selectively，diluting finitely，enzyme-linked immunosorbent assay (ELISA) and IFA test，to obtain the subtypes from the clone line by ELISA test.As a result，the cell lines secreted McAbs stably by culturing in vitro and recovery tests were obtained and named as 1D12，5H5 respectively，the ELISA titers of ascites was 1∶107，1∶105 respectively.The subtypes of the two McAbs were identified as IgG2b and IgM，Kappa light chain.The two McAbs reacted specifically with only matrix protein of PRRSV but not with PRV，PPV，PCV，CSFV，JEV by indirect-ELISA.The McAbs will be very useful as specific reagents in diagnosis and control of PRRS.

Key words: PRRSV; monoclonal antibody; matrix protein

CLC Number:

S852.65⁺9.6

WANG Yuan;HU Hong-hui;QIN Ya-man;ZHANG Qian;DENG Xiao-yu;CAO Zhen;WANG Chuan-bin;RAN Duo-liang;TIAN Ke-gong. Development of Monoclonal Antibodies against Matrix Protein ofPRRSV NVDC-JXA1 Strain[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(11): 70-73.

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Development of Monoclonal Antibodies against Matrix Protein ofPRRSV NVDC-JXA1 Strain

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