Abstract: A pair of cloning primers were designed based on the sequence information in silico and were used to clone and analysis the Sus scrofa thyroid hormone responsive spot14 (THRSP,accession: JF951726). The positive clone was identified and sequenced, at the same time the THRSP gene organization distribution rule was analyzed by RT-PCR. The length of Sus scrofa THRSP gene was 621 bp, including an open reading flame of 450 bp, encoding 150 amino acids. Identity analysis showed that the THRSP nucleotide sequence in Sus scrofa shared 83.8%, 88.5%, 80.9%, 80.7%, 85.1%, 47.1% homology with that of Homo sapiens, Bos taurus, Rattus norvegicus, Mus musculus, Oryctolagus cuniculus, Gallus gallus respectively, the predicted peptide shared 76.9%, 80.8%, 76.2%, 76.2%, 82.1%, 32.0% homology with that of Sus scrofa. The phylogenetic tree demonstrates that Sus scrofa was the closest to cattle. Semi-quantitative RT-PCR analysis showed that the expression level of THRSP was the highest in liver and adipose tissue, the lower in cerebrum, spleen, stomach, skin, caecum and rectum, the lowest in lung, duodena jejunum and ileum, without in kidney and muscle. The high expression level of THRSP in the liver, fat and other fat generation of the organization suggest that which might adjust as fat synthesis of metabolic control factor, but fat synthesis in the regulation mechanism needs further research.

Key words: Sus scrofa; thyroid hormone responsive spot14; clone; tissue expression