Abstract: 【Objective】 The purpose of this experiment was to evaluate the disinfection effect of the new full spectrum TiO₂ disinfectant and provide theoretical basis and reference for the clinical application of this disinfectant.【Method】 Through the laboratory disinfection test,the disinfection effect of full spectrum TiO₂ disinfectant was first investigated,and different bacterial liquids (Staphylococcus aureus,Bacillus subtilis,Pseudomonas aeruginosa and Escherichia coli) were coated and cultured for 24 h after spraying the disinfectant,and the growth status of the strains was observed to evaluate the preventive effect of the disinfectant on common bacteria.The disinfectant was sprayed immediately after the bacterial solution was coated on the medium,and the bactericidal rate was calculated at different time periods to investigate the bactericidal effect of the disinfectant on bacteria.Metal corrosion test tablets were immersed in disinfectants to assess the degree of erosion to clinical production equipment.The effect of light on bacteriostatic effect of disinfectant was also investigated.At the same time,clinical disinfection trials were carried out in a large-scale farm.The air and surface of the building were sampled and cultured before and after the use of the disinfectant,and the bactericidal effect of the disinfectant in clinical application was investigated by counting the total number of colonies,so as to comprehensively evaluate the disinfection effect and safety of the disinfectant.【Result】 In the laboratory disinfection evaluation, the results of prevention test showed that no bacteria colonized in the tested disinfectant group except Pseudomonas aeruginosa. The bactericidal test results showed that the test disinfectant could play a good sterilization effect within 10 min, the bactericidal rate of Staphylococcus aureus,Bacillus subtilis and Escherichia coli was over 99.80%.The bactericidal rate of Pseudomonas aeruginosa decreased after 6 h.The metal corrosion test results showed that the corrosion rate of 304 stainless steel under test for 72 h was 0.0047 mm/a,and there was basically no corrosion.The results of light test showed that there was no bacterial colonization in both the light avoiding and the non-light avoiding groups.In the clinical production investigation,compared with disinfectant control group,the total number of bacterial colonies in the air of hoggery in test disinfectant group was extremely significantly decreased (P<0.01),but the total number of bacterial colonies on the surface of objects had no significant difference (P＞0.05).【Conclusion】 The full spectrum TiO₂ disinfectant had a strong killing effect on common pathogens,and had a good performance in clinical efficacy evaluation.The bactericidal rate in the air was as high as 100%,and the use cost was low,which had a good application and development prospect,and provided a new scheme for the environmental disinfection of large-scale farms.

Key words: TiO₂; environmental disinfectant; sterilization; bacteriostasis