基于“桂芯一号”液相芯片的南丹瑶鸡鸡冠性状全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2025.04.024

Abstract

Abstract: 【Objective】 The aim of this study was to identify candidate gene and molecular marker that affected the comb trait of Nandan-Yao chickens,thus to provide technical support for the selection and breeding of comb traits in Nandan-Yao chickens.【Method】 In this study,phenotypes of comb traits were collected from 464 Nandan-Yao chickens at 7,10,15,18,22 and 26 weeks of age.Meanwhile,DNA were extracted from blood samples and genotyping was conducted using "GXChickChip-I" chip.Genome-wide association study (GWAS) were performed on the comb height,comb length,comb thickness and comb area of Nandan-Yao chickens at different ages by the univariate mixed linear model in GEMMA software.The gene annotated from the associated single nucleotide polymorphism (SNP) were subjected to GO function and KEGG pathway enrichment analysis.【Result】 A total of 7,8,21 and 10 SNPs were detected to be associated with comb area,comb height,comb length and comb thickness in Nandan-Yao chickens,respectively.These SNPs were annotated to 37 genes.Through bioinformatics analysis and gene functional annotation,7 genes of CELF2,MAP7,MAP3K5,AKT3,IFNGR1,IL20RA and MANBA were identified as candidate genes related to comb traits in Nandan-Yao chickens.【Conclusion】 This study revealed 39 potential SNPs associated with comb traits in Nandan-Yao chickens,and 7 genes that might influence comb size were identified.The results could provide effective molecular makers for the selection of comb traits of Nandan-Yao chickens and an important theoretical foundation for the breeding of excellent indigenous chickens.

Key words: Nandan-Yao chickens; comb traits; "GXChickChip-I" chip; genome-wide association study

CLC Number:

S831.2
Q78

YANG Zhuliang, LUO Jintang, ZHANG Zhen, LI Jianneng, LI Fuqiu, YANG Xiurong. Genome-wide Association Study of Comb Traits in Nandan-Yao Chickens Based on the "GXChickChip-I" Chip[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1716-1728.

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Genome-wide Association Study of Comb Traits in Nandan-Yao Chickens Based on the "GXChickChip-I" Chip

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