静原鸡DERA基因生物信息学分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2023.05.004

Abstract

Abstract: 【Objective】 The aim of this study was to analyze the sequence characteristics of 2-deoxyriboaldolase (DERA) CDS region and its expression levels in different tissues and different growth stages,so as to lay a foundation for further study of the regulatory mechanism of DERA gene in the muscle of Jingyuan chickens.【Method】 The complete CDS region of DERA gene was cloned,sequenced and bioinformatics analyzed using the breast muscle cDNA of 42 days of age chickens as template.Real-time quantitative PCR was used to detect the expression of DERA gene in chest muscle,leg muscle and different tissues (heart,spleen,liver,kidney,lung,chest muscle and leg muscle) of Jingyuan chickens at different growth and development stages(7,42,126 and 180 days of age).【Result】 The CDS region of DERA gene in Jingyuan chickens was 699 bp in length,encoding 232 amino acids,and had the highest similarity (99.1%) with Phasianus colch.The results showed that the amino acid sequence of DERA protein was highly conserved during the evolutionary process.DERA was a stable protein,the average hydrophilic coefficient was 0.175,which was a hydrophobic protein.DERA protein had a transmembrane structure without signal peptide,which was a transmembrane protein.The protein domain was a deoC/LacD family aldolase domain.There were alpha helix,extended chain and random coil in secondary structure of DERA protein. Protein interaction network analysis showed that DERA protein interacted with PGM2,TPI1,TKT and other proteins.DERA gene was expressed in kidney,chest muscle,leg muscle,heart,liver,lung and spleen,among which the expression of kidney and chest muscle were higher,which were significantly higher than that of other tissues (P＜0.05),and the expression of lung and spleen were lower,which significantly lower than that of other tissues (P＜0.05).The expression of DERA gene in chest muscle and leg muscle at 7 days of age were significantly higher than that at 42,126 and 180 days of age (P＜0.05).【Conclusion】 The complete CDS sequence of DERA gene in Jingyuan chickens was successfully obtained in this experiment.The expression of DERA gene in chest muscle and leg muscle of Jingyuan chickens at different stages of growth and development was the highest at 7 days of age,and the expression of DERA gene in kidney and chast muscle of Jingyuan chickens were higher at 42 days of age.The results provided a reference for further study on the mechanism of DERA gene in muscle development of Jingyuan chickens.

Key words: Jingyuan chickens; DERA gene; bioinformatics; expression

CLC Number:

WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.

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Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens

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