牛支原体新疆分离株P48基因克隆及分子特征分析

doi:10.16431/j.cnki.1671-7236.2021.02.007

Abstract

Abstract: The aim of this study was to analyze the sequence characteristics of P48 gene of Mycoplasma bovis isolated strain in Xinjiang,and the structure and function of its encoded protein.Specific primers were designed based on the sequence of P48 gene of PG45 strain (GenBank accession No.:CP002188.1),the sequence of P48 gene was amplified by Overlap-PCR.P48 gene was inserted into pMD19-T vector for cloning and sequencing.Bioinformatics methods were used to predicted and analyzed its nucleotide sequences and amino acid sequences,including the basic physicochemical properties,signal peptide,transmembrane,phosphorylation site,glycosylation site,secondary structure,tertiary structure.The results showed that the sequence length of Mycoplasma bovis isolated strain P48 gene was 1 407 bp,and its homology with Mb PG45 international standard strain was 100%,and it was clustered in one strain.The P48 protein encoded a protein composed of 467 amino acids,no transmembrane structure,which was a stable soluble hydrophilic protein.The P48 protein had a signal peptide,44 potential phosphorylation sites and 3 glycosylation sites.The secondary structure was mixed,and the alpha helix was the most,accounting for 41.76%,followed by the random coil,accounting for 37.69%.Functional prediction showed that the P48 protein had high probability in signal transduction,stress response and receptor.In this study,the isolated strain P48 gene was cloned successfully,and the protein that it encoded was a stable soluble hydrophilic protein.The results provided a theoretical basis for further exploration of its function of P48 gene of Mycoplasma bovis isolated strain.

Key words: Mycoplasma bovis; P48 gene; cloning; bioinformatics analysis

CLC Number:

S828

LING Chen, HAO Chengwu, ZHANG Fei, HOU Feng, HE Sun. Cloning and Bioinformatics Analysis of P48 Gene of Mycoplasma bovis Isolated Strain in Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 457-466.

References

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Cloning and Bioinformatics Analysis of P48 Gene of Mycoplasma bovis Isolated Strain in Xinjiang

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