静原鸡miR-107的靶基因预测及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.09.001

Abstract

Abstract: To explore the regulatory mechanism of miR-107 in the process of inosine acid specific deposition of muscle tissue in Jingyuan chickens,the bioinformatics software and databases were used to bioinformatically analyze and predict target genes of miR-107 in Jingyuan chickens in this study.The transcriptome sequencing technology was used to obtain the sequence of miR-107 in Jingyuan chickens,perform sequence alignment and analyze its conservation.TargetScan,miRDB and PicTar were applied to predict target genes of miR-107.The intersection of the three results and validated targets from miRTarbase database were demonstrated by Gene Ontology and signal pathway enrichment analysis using OmicShare tool,and discussed the expression level of miR-107 in different tissues and diseases in HMED database.The results showed that miR-107 sequence of Jingyuan chickens was highly conserved among all species,and the biological functions of target genes regulated by miR-107 mainly included basic biological function,such as cellular processes,biological regulation,metabolic processes,response to stimulus and molecular functions were enriched in binding,catalytic activity,transcription regulator activity and so on.Target genes existed in various components of the cell,including organelles,cell membranes and protein-containing complexes.The signal transduction pathway was significantly enriched in the microRNAs in cancer,human papillomavirus infection,pathways in cancer and PI3K-Akt signaling pathway (P<0.05).miRNA-target gene interaction analysis found that miR-107 mainly inhibited the expression of target genes through targeted binding to the untranslated region of the target gene mRNA.According to the expression data of miR-107 in different tissues and diseases in the HMED database,it was found that the expression level of miR-107 was the highest in melanoma,and was lower in tonsil,muscle and other tissues.The prediction and bioinformatics analysis of miR-107 target genes provided a reference for in-depth study of the function and regulatory mechanism,and laid the basis to study the role of miR-107 of muscle development in Jingyuan chickens.

Key words: Jingyuan chickens; miR-107; target gene; bioinformatics

CLC Number:

YU Baojun, HE Jintong, YAO Tingting, WU Hao, FAN Xiya, HU Honghong, WANG Weizhen, GUO Ju, GU Yaling, CAI Zhengyun, XIN Guosheng, ZHANG Juan. Prediction and Bioinformatic Analysis of miR-107 Target Genes in Jingyuan Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2695-2705.

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Prediction and Bioinformatic Analysis of miR-107 Target Genes in Jingyuan Chickens

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