›› 2019, Vol. 46 ›› Issue (2): 621-627.doi: 10.16431/j.cnki.1671-7236.2019.02.034

• Basic Veterinary Medicine • Previous Articles     Next Articles

Establishment and Application of Fluorescence Quantitive Immunochromatographic Assay for Canine C-reactive Protein

KANG Ye1, WANG Yu1, HU Shukun1, WU Peidian2, LI Wenmei1,2, HE Xiaowei1   

  1. 1. College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China;
    2. Guangzhou Wondfo Biotech Co., Ltd., Guangzhou 510663, China
  • Received:2018-08-15 Online:2019-02-20 Published:2019-02-20


The study focused on the establishment of fluorescent microsphere immunochromatography quantitative method of canine C-reactive protein (C-CRP) to a develop test strips of simple operation and high sensitivity for rapid quantitative detection of C-CRP.A fluorescent sandwich immunoassay via double antibody was set up,anti-C-CRP monoclonal antibody and goat anti-chicken IgY labeled with carboxy fluorescent microspheres were used as labeled antibody,the other anti-C-CRP monoclonal antibody and chicken IgY were used as capture antibody for detection lines and control line.The results showed that the detection range of the lateral flow assay was 0.5 to 250 mg/L,the detection limit was 0.5 mg/L,and the CV in intra-assay and inter-assay were 0.68% to 6.94% and 0.89% to 8.79%,respectively.The average recovery was 98.15% to 101.19%,and there was no cross-react with 9 interfering substances.The stressed condition stability test showed that the test strip could be stored for 24 months at room temperature.For clinical sample test,the test strips had a good correlation with the control reagent (R2=0.995).In conclusion,the prepared fluorescent immunochromatographic strips could be applied to clinical test of C-CRP concisely and conveniently.

Key words: canine C-reactive protein(C-CRP); fluorescence immunochromatography; quantitative detection

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