›› 2014, Vol. 41 ›› Issue (9): 145-149.

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The Optimization of Primary Hemocyte Culture of Scylla paramamosain

QIAO Kun1, ZHANG Ya-qun1, WANG Shu-ping1, AN Zhe1, HAO Hua1,2, CHEN Fang-yi1,2, PENG Hui1,2   

  1. 1. College of Ocean and Earth Science, Xiamen University, Xiamen 361102, China;
    2. Fujian Engineering Laboratory of Marine Bioproducts and Technology, Xiamen 361102, China
  • Revised:2014-07-02 Online:2014-09-20 Published:2014-09-24

Abstract: The optimization of primary hemocyte culture of Scylla paramamosain was successfully studied in vitro.The results showed that the hemocyte cells were morphologically kept activity better at pH 4.6 marine crustaceans anticoagulant.High concentration of fetal bovine serum (>15%) in the culture lead to development of vacuoles.The hemocyte cells survived best in L15 medium supplemented with 5% fetal bovine serum and 1.2% sodium chloride.The house keeping gene β-actin of Scylla paramamosain of cultured hemocyte maintained stably expressed through the experiment.This cell culture system could be used for in vitro experiments.

Key words: Scylla paramamosain; hemocyte; primary culture

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