福氏志贺菌2型多重PCR检测方法的建立

Abstract

Abstract: In order to build a fast, efficient and specific multiplex PCR method for detection and identification of Shigella flexneri， this study used specific genes ipaH, gtrⅡ, gtrⅩ and R002 in different serotypes of Shigella to design the corresponding primers to amplify the target fragment, and in the same PCR system validated the Shigella flexneri. At the same time through a series of exploration and adjustments to optimize the reaction conditions, we established a method to detect Shigella flexneri type 2a and 2b out of a variety of bacteria quickly. The results suggested that the multiplex PCR could identificate the Shigella flexneri quickly, efficiently and accurately, and had great significance for the prevention of laboratory contamination， clinical diagnosis and treatment of shigellosis.

Key words: multiple PCR; Shigella flexneri; specific genes; primers

ZHU Zhen, WANG Jing, ZHANG Ji-yu, WEI Xiao-juan, ZHOU Xu-zheng, GUO Xiao, LIU Cui-cui. Establishment of Multiplex PCR for Detecting of Shigella flexneri Serotype 2[J]. .

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Establishment of Multiplex PCR for Detecting of Shigella flexneri Serotype 2

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