小反刍兽疫病毒Tibet 07株F蛋白在昆虫细胞中的表达及其抗原性鉴定

Abstract

Abstract: In order to obtain purified peste des petits ruminants virus F protein, the F gene of peste des petits ruminants virus strain Tibet 07 was cloned into transfer vector pFastBac/CT-TOPO, plasmid pFastBacCT-PPRV-F was then transformed into DH10Bac complement cells. The recombinant bacmid DNA was isolated and transfected into sf9 insect cells to express F protein. The expressed F protein was identified by using SDS-PAGE, Western blotting and indirect immunofluorescence assay. The recombinant F protein was successfully expressed in insect cells with a relative molecular of 59 ku.The sf9 cells infected by Bacmid-PPRV-F can reacted with positive serum of peste des petits ruminants.Using the Bac-to-Bac baculovirus expression system expressed the F protein of peste des petits ruminants virus strain Tibet 07.This work provides basis for development of rapid test for detection of peste des petits ruminants antibody.

Key words: peste des petits ruminants; F protein; baculovirus expression system

ZHAO Wen-nian, WANG Qing-hua, LIU Chun-ju, WANG Shu-juan, LEI Cheng-hong, BAO Jing-yue, WANG Zhi-liang. Expression and Identification of F Protein of Peste Des Petits Ruminants Virus Strain Tibet 07 in Insect Cells[J]. .

References

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Expression and Identification of F Protein of Peste Des Petits Ruminants Virus Strain Tibet 07 in Insect Cells

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