山羊支原体山羊肺炎亚种单克隆抗体的制备及抗体结合抗原表位的筛选

Abstract

Abstract: The aim of this research was to prepare monoclonal antibodies against Mycoplasma capricolum subsp.capripneumoniae (M.capricolum subsp.capripneumoniae, Mccp), and also screen antibody binding epitopes. We inactivated Mccp with formaldehyde, and then immunized BALB/c mice. We applied conventional fusion cell technology to obtain hybridoma cell, subclones, and ascites of monoclonal antibodies. We used enzyme-linked immunoassay (ELISA) and immunoblotting (Western blotting) technologies to identify the specificity of monoclonal antibodies, and enzyme digestion method to identify antibody binding epitopes. Finally, we successfully screened five monoclonal antibodies and identified three antibody binding epitopes, which provided a preliminary and reliable experimental basis for further scientific research.

Key words: Mycoplasma capricolum subsp.capripneumoniae; monoclonal antibodies; antibody binding epitopes

ZHENG Ying-ying, PIAN Ya-ya, CHU Yue-feng, ZHAO Ping, HE Ying, JIAN Ying-na, JIANG Yong-qiang, LU Zhong-xin. Preparation of Monoclonal Antibodies against Mycoplasma capricolum subsp. capripneumoniae and Screening of Antibody Binding Epitopes[J]. China Animal Husbandry & Veterinary Medicine.

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Preparation of Monoclonal Antibodies against Mycoplasma capricolum subsp. capripneumoniae and Screening of Antibody Binding Epitopes

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