饲料中牛、羊源性成分现场可视化检测方法的建立

Development of Visual Loop-mediate Isothermal Amplification Assay for Bovine and Ovine-derived Materials in Feedstuff

ZHU Zhu¹，LAN Qing-kuo¹，ZHAO Xin¹^，2，CHEN Rui¹，GUO Yong-ze¹，WANG Yong¹^，3

1.Tianjin Institute of Agricultural Quality Standard and Testing Technology，Tianjin 300381，China；2.School of Chemical Engineering，Tianjin University，Tianjin 300072，China；3.College of Plant Protection，Hebei Agricultural University，Baoding 071001，China

Received:2014-01-22 Online:2014-07-20 Published:2014-08-21

Abstract

Abstract: Two set of primers were designed according to the mitochondrial DNA sequences of the targets，8 meat powder were detected by visual loop-mediate isothermal amplification （vLAMP） for method specificity，and meanwhile the mode of artificially contaminated feed was constructed to evaluate the sensitivity of vLAMP assay.The results showed that the bovine and ovine-derived materials had specific amplification，but the other animal origin submitted negative reactions.Sensitivity of vLAMP assay for bovine and ovine-derived materials was 0.001%.In conclusion，the vLAMP assay developed in the present study was a specific，sensitive，simple and convenient method for the rapid screening of bovine and ovine-derived materials in contaminated feeds.

Key words:

bovine and ovinemso-bidi-font-family: 宋体" lang="EN-US">-derived materials；loopmso-bidi-font-family: 宋体" lang="EN-US">-mediated isothermal amplification；visualization technology

ZHU Zhu，LAN Qing-kuo，ZHAO Xin，CHEN Rui，GUO Yong-ze，WANG Yong. Development of Visual Loop-mediate Isothermal Amplification Assay for Bovine and Ovine-derived Materials in Feedstuff[J]. .

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Development of Visual Loop-mediate Isothermal Amplification Assay for Bovine and Ovine-derived Materials in Feedstuff

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