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Establishment of Indirect ELISA Detection Method for Toxoplasma gondii Antibody in Dogs

JIA Yan1,CHEN Yu-huan2,YANG Li-na2,LIU Yue-sheng1   

  1. 1. Jiaxing Vocational Technical College,Jiaxing 314036,China;2. Beijing Peking Biological Technical Limited Company,Beijing 102200,China

  • Revised:2014-05-13 Online:2014-07-20 Published:2014-08-21

Abstract: The assay was aimed to establish a rapid and specific indirect ELISA method for canine Toxoplasma gondii antibody detection,using expressed protein of recombinant Toxoplasma gondii SAG1 gene transfected into Leishmania tarentolae as the coating antigen. The optimal antigen coating concentration was 6.75 μg/mL, serum optimal dilution was 1∶100, the known positive serum detection was more than 1∶6400, the coefficients of variation for intra-assay and inter-assay were both less than 10%, and the coated ELISA plate could be stored more than 8 months at 4 and -20 ℃. The present indirect ELISA detection method was sensitivity and specificity for detecting Toxoplasma gondii antibody in canine.

Key words:

canine; Toxoplasma gondii antibody; indirect ELISA