犬弓形虫抗体间接ELISA检测方法的建立

China Animal Husbandry & Veterinary Medicine

Previous Articles Next Articles

Establishment of Indirect ELISA Detection Method for Toxoplasma gondii Antibody in Dogs

JIA Yan¹,CHEN Yu-huan²,YANG Li-na²,LIU Yue-sheng¹

1. Jiaxing Vocational Technical College,Jiaxing 314036,China;2. Beijing Peking Biological Technical Limited Company,Beijing 102200,China

Revised:2014-05-13 Online:2014-07-20 Published:2014-08-21

Abstract

Abstract: The assay was aimed to establish a rapid and specific indirect ELISA method for canine Toxoplasma gondii antibody detection，using expressed protein of recombinant Toxoplasma gondii SAG1 gene transfected into Leishmania tarentolae as the coating antigen. The optimal antigen coating concentration was 6.75 μg/mL, serum optimal dilution was 1∶100, the known positive serum detection was more than 1∶6400, the coefficients of variation for intra-assay and inter-assay were both less than 10%, and the coated ELISA plate could be stored more than 8 months at 4 and -20 ℃. The present indirect ELISA detection method was sensitivity and specificity for detecting Toxoplasma gondii antibody in canine.

Key words:

canine; Toxoplasma gondii antibody; indirect ELISA

JIA Yan,CHEN Yu-huan,YANG Li-na,LIU Yue-sheng. Establishment of Indirect ELISA Detection Method for Toxoplasma gondii Antibody in Dogs[J]. China Animal Husbandry & Veterinary Medicine.

References

1 于恩庶.弓形虫病学［M］.福建:福建科学技术出版社，1992.
2 马浩杰，王惠玲，周永华，等.弓形虫感染流产孕妇血清TNF-α水平分析［J］.中国血吸虫病防治杂志，2007，19(5)：371～373.
3 王崇功，周永华，吴菁，等.弓形虫感染对优生优育危害性及其控制的研究［J］.中国血吸虫病防治杂志，1998，10(6)：362～365.
4 任科研，李琳，苑淑贤，等. 弓形虫PCR检测试剂盒的组装和初步应用［J］.中国畜牧兽医，2012，39(9)：92～94.
5 许越，杨欢欢，魏峰，等.弓形虫SAG1基因在蜥蜴利什曼原虫中的表达［J］.中国兽医学报，2012，5(32)：696～700.
6 何艳燕, 蒋守富, 马杏宝, 等. 国内外16种弓形虫诊断试剂盒的评估［J］. 旅行医学科学, 2008(3)：43～45.
7 何勇，朱艳平，袁子国，等.几种检测猪弓形虫病ELISA诊断试剂盒的对比［J］.中国畜牧兽医，2011，38(6)：218～221.
8 张居作，陈汉忠，徐君飞. 我国弓形虫的感染现状［J］.动物医学进展，2008，29(7)：101～104.
9 郭志红，张艳萍，王威.如何防制人畜共患寄生虫病［J］.中国动物检疫，2009，26(4)：69～71.
10 徐大纲.弓形虫P30基因及其功能［J］.中国兽医寄生虫病，2002，10(3)：42～45.
11 Ajzenberg D.Unresolved questions about the most successful known parasite［J］.Expert Rev Anti Infect Ther，2011，9(2)：69～71.
12 Clayton C，Shapira M.Post-transcriptional regulation of gene expression in trypanosomes and leishmanias［J］.Mol Bioehem Parasitol，2007，156(614)：93～101.
13 Guglietta S，Beghtto E，Spadoni A，et al.Age-dependent impairment of functional helper T cell responses to immunodominant epitopes of Toxoplasma gondii antigens in congenitally infected individuals［J］.Microbes Infect，2007，9(2)：127～133.
14 Hill D E, Chirukandoth S, Dubey J P, et al. Comparison of detection methods for Toxoplasma gondii in naturally and experimentally infected swine［J］. Vet Parasitol, 2006, 141(1～2): 9～17.
15 Kushnir S，Cirstea I C，Basiliya L，et al.Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae［J］.Mol Biochem Parasitol，2011，176(2)：69～79.

Establishment of Indirect ELISA Detection Method for Toxoplasma gondii Antibody in Dogs

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments