核酸免疫制备抗猪流感病毒HA单克隆抗体

Abstract

Abstract: For the preparation of monoclonal antibody(McAb) against HA protein of swine influenza virus, DNA plasmid bearing HA gene of A/Swine/Guangdong/2004(H1N1) was used to immunize BALB/c mice followed by generation of a McAb using the hybridoma technique. The generated nine McAbs were identified by ELISA. Functional analysis demonstrated the McAb 4D5 neutralized the virus. Furthermore, the McAb 4D5 didn’t have cross-reactivity with H3N2 or H5N1 influenza viruses, showing highly specific to H1N1. Immunofluorescence assay showed that McAb 4D5 was able to detect infection of cells with H1N1. Over all, McAb 4D5 proved specific and highly sensitive for A/Swine/Guangdong/2004(H1N1) HA and could potentially serve as immunodiagnostic tools for the rapid detection of this specific strain of influenza virus and laid the foundation for the study of single chain antibody fragment.

Key words: H1N1 influenza virus; hemagglutinin protein; monoclonal antibody

LI Yue, WANG Wei, ZHAO Ying-hui, ZHOU Chang-liang, ZANG Feng-xia, RAN Duo-liang, MENG Qing-wen, CHEN Hong-yan. Preparation of Monoclonal Antibody against Swine Influenza Virus HA by Nucleic Acid Immunization[J]. .

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Preparation of Monoclonal Antibody against Swine Influenza Virus HA by Nucleic Acid Immunization

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