山东省肉鸭沙门氏菌的分离鉴定及敏感抗菌药物的筛选

Isolation and Identification of Duck Salmonella and Screening of Sensitive Antimicrobials in Shandong Province

QI Jing¹, WANG Shou-rong², LI Lan-bo³^，4, LUO Yan-bo¹, LI Lu-lu¹, HU Ming¹, WANG Jin-wen¹, LI Hong-da¹, JIA Ji-mei¹, ZHAO Min¹, YANG Sheng-fu¹, LI Yu-bao², SHAN Hu⁴, LIU Yu-qing¹^，4

1. Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2. College of Agriculture, Liaocheng University, Liaocheng 252059, China; 3. Experimental Animal Center of Shandong Province, Shandong Academy of Medical Sciences, Jinan 250002, China; 4. Shandong Key Laboratory of Preventive Veterinary Medicine, Shandong Engineering Research Center of Veterinary and Diagnostic Reagents, Qingdao Agricultural University, Qingdao 266109, China

Revised:2014-03-31 Online:2014-07-20 Published:2014-08-21

Abstract

Abstract: 317 tissue samples of sick and dead ducks from various regions of Shandong province were collected for bacterial isolation, staining microscopy and biochemical identification. Totally, 105 strains of Salmonella were identified and the separation rate was 33.1%. Antimicrobial susceptibility testing of all isolates to 17 kinds of antibiotics was carried out by setting up different concentration gradient （1/4 L, 1/2 L, L, 2 L and 4 L） of the clinical recommended dose. The sensitive antimicrobials to the isolates were screened according to the inhibition ratio greater than 80% at the concentration of L gradient. The result showed that Salmonella was one of the main pathogenic bacteria in duck breeding of Shandong province, and with severe multiple drug resistance. Several sensitive antimicrobials including ampicillin, norfloxacin, kanamycin, florfenicol and pefloxacin could be used as clinical recommended drugs for the prevention and control of this disease.

Key words:

Salmonella; isolation; identification; antimicrobial susceptibility testing; duck

QI Jing， WANG Shou-rong, LI Lan-bo LUO Yan-bo, LI Lu-lu, HU Ming, WANG Jin-wen, LI Hong-da, JIA Ji-mei, ZHAO Min, YANG Sheng-fu, LI Yu-bao, SHAN Hu, LIU Yu-qing. Isolation and Identification of Duck Salmonella and Screening of Sensitive Antimicrobials in Shandong Province[J]. .

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Isolation and Identification of Duck Salmonella and Screening of Sensitive Antimicrobials in Shandong Province

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