小型猪克隆胚胎体外培养阶段的优化

Abstract

Abstract: To improve the in vitro maturation of pig oocytes and to modify the protocols of cloning, the size of cumulus-oocyte and timing of the first zygotic cleavage were mainly studied in the present study. The oocytes were divided into three groups by the number of cumulus cells, subsequently the maturation efficiency was compared, and the matured oocytes were used for the following experiments. According to timing of the first zygotic cleavage, PA and SCNT embryos were divided into three groups to compare the rates of cleavage and blastocysts in the in vitro culturing. Our results showed that a better ability of maturation was observed in the oocytes which had more cumulus cells(39 to 40 h) and few cumulus cells(41 to 42 h) than those oocytes of which the number of cumulus cells(39 to 42 h) didn’t being distinguish. Both of PA and SCNT, the embryos cleaved in 26 h had a better developmental competence compared to the embryos cleaved after 26 h, which was evaluated by the rates of cleavage and blastocysts. Therefore, in the present study, not only the miniature porcine cloning programs was improved, but also that it provided technical reference for organ xenotransplantation.

Key words: miniature porcine; xenotransplantation; timing of the first zygotic cleavage; SCNT

YANG Xi-xiang, LI Zhi-fang, LONG Chuan, FENG Chong, CHU Ming-xing, PAN Deng-ke, LI Juan. Optimization of Miniature Porcine Cloning Embryos in vitro Stage[J]. .

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Optimization of Miniature Porcine Cloning Embryos in vitro Stage

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