胰岛素样生长因子-Ⅰ基因与京海黄鸡生长和繁殖性状的关联分析

Genetic Effects of IGF-Ⅰ Gene on the Growth and Reproductive Traits in Jinghai Yellow Chicken

ZHAO Xiu-hua¹^，2, LI Man-yu¹, WANG Jin-yu², LIU Guo-jun¹

1.Animal Husbandry Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China；2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2013-12-16 Online:2014-07-20 Published:2014-08-21

Abstract

Abstract: The aim of this study was to analyze the association of SNPs in insulin like factor-Ⅰ（IGF-Ⅰ） gene with chicken growth and reproductive traits. PCR-SSCP approach was applied to assess the single nucleotide polymorphisms(SNPs) and the general linear model was used to analyze genetic effects between genotypes and growth traits of the Jinghai Yellow chicken. For the IGF-Ⅰ gene, three genotypes(AA, AB and BB) were detected in Jinghai Yellow chicken population, the frequency of A and B alleles were 0.613 and 0.387. Sequencing revealed one mutation(A60G) of IGF-Ⅰ gene in BB genotype in comparison to AA genotype. The results showed that BB genotype of the IGF-Ⅰ gene had higher bodyweight at 4 weeks compared to AA and AB genotypes(P<0.05)；AA genotype of the IGF-Ⅰ gene had significantly higher bodyweight at 300 day-age-weight compared to BB genotype(P<0.05). Polymorphisms in exon 3 of IGF-Ⅰ gene had certain effect on growth and reproductive traits in Jinghai Yellow chicken. However, whether these polymorphisms were important genetic markers, which related with IGF-Ⅰ gene and reproduction traits in Jinghai Yellow chicken, were needed to be further studied.

Key words:

Jinghai Yellow chicken；IGFmso-bidi-font-family: 宋体" lang="EN-US">-mso-bidi-font-family: 宋体">Ⅰ gene；PCRmso-bidi-font-family: 宋体" lang="EN-US">-SSCP；polymorphism；growth traits；reproductive traits

ZHAO Xiu-hua, LI Man-yu, WANG Jin-yu, LIU Guo-jun. Genetic Effects of IGF-Ⅰ Gene on the Growth and Reproductive Traits in Jinghai Yellow Chicken[J]. .

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Genetic Effects of IGF-Ⅰ Gene on the Growth and Reproductive Traits in Jinghai Yellow Chicken

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