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Drug-resistance Analysis of Coagulase-negative Staphylococcus Isolated from Dairy Cow Mastitis

WANG Xin-yu1,PEI Lin1,YU En-qi1,LIN Wei-dong1,WEN Xin-tong1,ZAI Xu-sheng1,QIN Ai-jian1,JIN Wen-jie1,ZHANG Jun2,LIANG Xiang-huan2,WANG Jin-rong2,HANG Bo-lin1,3   

  1. 1.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Key Laboratory for Animal Preventive Medicine of Jiangsu Province,College of Veterinary Medicine, Yangzhou University, Yangzhou 225009,China;2.Farm of Experiment,Yangzhou University,Yangzhou 225009,China;3.College of Animal Science,Henan Institute of Science and Technology,Xinxiang 453003,China)

  • Received:2013-12-05 Online:2014-06-20 Published:2014-07-24

Abstract: In this study, pathogenic epidemiology of subclinical mastitis in part of Jiangsu province dairy farms was investigated. It included sampling, bacteria cultivation, gram staining, microscopy observation and biochemical identification. 35 strains of coagulase-negative Staphylococcus (CNS) were isolated. Results of drug sensitivity test showed that 60% to 80% of CNS isolates were highly sensitive to clinical commonly used antibiotics like vancomycin, clarithromycin, benzene azole pennicillin, norfloxacin, chloromycetin, ciprofloxacin, cefotaxime, fosfomycin and nitrofurantoin. While for penicillin G, SMZ and polymyxin B, these isolates showed high drug resistance, the resistance ratio was 70% to 100%. All these CNS isolates showed multi-drug resistance, 3 CNS isolates were resistant to 20 drugs, 1 was resistant to 15 drugs,1 was resistant to 14 drugs,62.9% of these CNS isolates were resistant to more than 5 tested drugs. All these results indicated that the resistant status of CNS caused subclinical mastitis was very serious.

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