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Comparison of Three ELISA Kits for the Detection of Porcine Reproductive and Respiratory Syndrome Virus Antibody and its Application

HE Hai-jian1, XING Gang2，3, MA Tao3, QIU Xiao-huo3, JIANG Chun-yan1, ZHOU Cai-qin4   

  1. (1. College of Agriculture and Biotechnology, Jinhua Poletechnic, Jinhua 321000,China；2. Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China；3. Zhejiang Tongdian Biotechnology Co., Ltd., Hangzhou 310030, China；4. Zhejiang Centre of Animal Disease Control, Hangzhou 310020, China)

  • Revised:2014-04-04 Online:2014-06-20 Published:2014-07-24

Abstract: To comparatively analyzed piglets maternal antibodies and the antibody dynamics in immunized pigs using three different of antibody detection Kits of porcine reproductive and respiratory syndrome virus (PRRSV)，in order to provide selection refereace for Kits of monitoring PRRSV antibodies of pigs. The results showed that the Kappa value =0.784＞0.6 between Kit A and Kit C，the compared results were highly-consistent；the Kappa value was 0.422 between Kit B and Kit A，the Kappa value was 0.374 between Kit B and Kit C.Maternal antibody monitoring showed that PRRSV maternal antibodies of three Kits had become negative at 42 days old. PRRSV associated antibodies of using Kit A and Kit C were detected after immuned one week, three weeks to reach the peak, while the PRRSV antibodies of using Kit B were detected three weeks after immunization, and reached the peak in five weeks. This study provided the experimental basis for the three Kits were used to antibody surveillance and immune assessment in the clinical PRRSV.

Key words:

porcine reproductive and respiratory syndrome （PRRS）; indirect ELISA; comparison; immune antibody