牛病毒性腹泻病毒非结构蛋白NS3的表达及ELISA检测方法的建立

Abstract

Abstract: An indirect enzyme-linked immunosorbent assay (ELISA) method was developed to detect antibody against bovine viral diarrhea virus (BVDV). The nonstructural protein NS3 gene of BVDV was subcloned into prokaryotic vector pET-32a to express. The recombinant protein NS3 of BVDV was used as the coating antigen for the ELISA method, and the reaction conditions were optimized. The specificity, sensitivity and reproducibility all showed good results. Furthermore, four hundred and seventy five bovine serum samples, which had been collected from several cattle farms of Guangxi, were detected by the NS3-ELISA, and the positive rate of BVDV antibody was 24.8%. Compared with commercial kit, the coincidence was 97%. The result suggested that NS3-ELISA was a convenient, rapid method for the diagnosis, antibody level surveillance and epidemiologic investigation of BVDV.

Key words:

bovine viral diarrhea virus; NS3; ELISA

FAN Qing, XIE Zhi-xun, XIE Zhi-qin, LIU Jia-bo, PANG Yao-shan, DENG Xian-wen, XIE Li-ji, LUO Si-si. Expression of Nonstructural Protein NS3 and Establishment of ELISA Method of Bovine Viral Diarrhea Virus[J]. .

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Expression of Nonstructural Protein NS3 and Establishment of ELISA Method of Bovine Viral Diarrhea Virus

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