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Isolation and Identification of Haemophilus parasuisand Correlative Research

WANG Xue-min1,2,LU Ming-hua1,3,CHEN Xiao-ling3,LU Ying-ying1,3,ZHANG Pei-jun3,GONG Yu-mei3,WANG Hong-jun3   

  1. (1. College of Agriculture, Hebei University of Engineering, Handan 056021, China; 2. Poultry Diseases Engineering Technology Research Center of Hebei Province, Handan 056021, China; 3. Beijing Municipal Key Laboratory of Animal Disease Prevention and Control Technology, Beijing Institution of Animal Husbandry and Veterinary Medicine, BAAFS, Beijing 100097, China)
  • Revised:2014-02-18 Online:2014-05-20 Published:2014-06-25

Abstract: In 2013, nineteen small gram-negative bacilli were isolated from pigs with suspected infection of Haemophilus parasuis in Beijing. Isolates were identified as Haemophilus parasuis with the culture characteristics, capsule staining, biochemical method, serotype and PCR identification, and these strains belonged to 4, 5, 7, 12, 13 serotypes. The results of the drug sensitivity test and the pathogenicity test of the nineteen isolates showed that the isolates were multi-drug resistance, and all strains except GS-3 had strong toxicity.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Haemophilus parasuisfont-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">; isolation and identification; drug resistance; virulence