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Isolation and Identification of Haemophilus parasuis, Study on its Inactivated Bacterin

LI Xiao-hua1,2, LIN Biao-sheng1,2, LIU Jian-kui1,2, DAI Ai-ling1,2, YANG Xiao-yan1,2   

  1. （1.College of Life Sciences,Longyan University,Longyan 364000,China；2.Fujian Key Laboratory of Preventive Veterinary Medicine and Biotechnology,Longyan 364000,China）
  • Received:2013-09-09 Online:2014-03-20 Published:2014-05-15

Abstract: A total of 28 Haemophilus parasuisc （H.parasuis） isolates were isolated from 48 farms in Longyan. Strains were confirmed by biochemical tests and PCR. Serovar 5 was the most prevalent, followed by serovar 4. 14 days old piglets were immunized with inactivated bacterin using seorvar 4 isolate LY04 and seorvar 5 isolate LY052, respectively, which were representative strains, and the results of efficacy test showed that the protection rates were both 66.7%.

Key words: Haemophilus parasuis; isolation and identification; inactivated bacterin