Abstract: In this study, Cashmere goat skin by extracting total RNA, cloning keratin-associated protein 1.1 (KAP1.1) gene eukaryotic expression vector pEGFP-N3-KAP1.1, using liposome-mediated recombinant plasmids were transformed into Inner Mongolia Cashmere goats cultured fibroblasts, temporal and spatial expression of the genes in eukaryotic cells, subcellular localization and expression pattern. The results showed that 2.0 μg plasmid and 6.0 μL ratio liposomes suitable for the optimization of the experimental protocol, KAP1.1 gene could be successfully expressed in fibroblasts in Inner Mongolia Cashmere goat, and 48 h after transfection, the transfection efficiency reaches a peak, the gene in eukaryotic cells in vitro on the nucleus, cytoplasm and cell membrane are expressed, but the strongest expression of the nucleus.

Key words: Inner Mongolia Cashmere goats; KAP1.1 gene; vector construction