猪TIGAR mRNA SYBR GreenⅠ荧光定量RT-PCR方法的建立

Abstract

Abstract: According to swine TIGAR mRNA sequences available in GenBank （No：XM_001926543）, a pair of primers was designed, with experimental condition optimizing, the method of a Real-time fluorescent quantitative reverse transcription-polymerase chain reaction （RT-PCR） was established to detect swine TIGAR gene, and the level of TIGAR mRNA of Landrace in different slaughter time was compared with the method established.The results showed that the R² of standard curves of TIGAR and the housekeeper gene β-actin were 0.9995 and 0.9996, respectively, and the amplification efficiency of TIGAR （103.7%） was consistent with β-actin （104.1%）.The methods established can be used for the determination of TIGAR mRNA in swine with high specificity and sensitivity. The research result was valuable to application.

Key words: swine; TIGAR mRNA; SYBR GreenⅠ; RT-PCR

KANG Da-cheng, TANG Xiao-yan, LI Peng-ying, YAN Cheng-ying, FAN Cheng-ming. Development of SYBR GreenⅠ-based RT-PCR Assay for Detection of TIGAR mRNA in Swine[J]. .

References

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Development of SYBR GreenⅠ-based RT-PCR Assay for Detection of TIGAR mRNA in Swine

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