伊氏锥虫变异表面糖蛋白抗原双抗体夹心ELISA检测方法的建立及初步应用

Abstract

Abstract: A double antibody sandwich ELISA for detection of Trypanosoma evansi variant surface glucoprotein (VSG) antigen was established by using two monoclonal antibodies against Trypanosoma evansi VSG antigen.The result of sensitivity showed when positive serum was diluted to 3200 doubles， its D_{450 nm} value was still greater than the critical value of positive and negative,and specificity test result showed that there was no cross reaction with the antigens of Theileria,Toxoplasma gondii,Chlamydia and Fasciola gigantica.82 clinical sera were detected by this double antibody sandwich ELISA and its positive rate was 9.76%.The experiment results demonstrated that this double antibody sandwich ELISA for detection of Trypanosoma evansi VSG antigen had good detecting sensitivity and specificity,and could be used as an effective method for clinical detecting buffalo Trypanosoma evansi disease.

Key words: Trypanosoma evansi; variant surface glucoprotein （VSG） antigen; monoclonal antibody; double antibody sandwich ELISA

ZHANG Ya-ling,WANG Kai,CHEN Fang-ling,ZENG Xiao-fei,WU Wen-de, HUANG Tian,LI Xue,ZHU Bing-lun,CHEN Han-zhong. Development and Preliminary Application of Double Antibody Sandwich ELISA for Detection of Trypanosoma evansi VSG Antigen[J]. .

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Development and Preliminary Application of Double Antibody Sandwich ELISA for Detection of Trypanosoma evansi VSG Antigen

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