猪圆环病毒2型流行毒株FF株的分离与鉴定

Abstract

Abstract: The materials from the clinical samples of kidney and inguinal lymph nodes characterized by postweaning multisystemic wasting syndrome (PMWS) of the pigs,proved to be porcine circovirus type 2 （PCV2） positive samples through the amplification of ORF2 gene of PCV2, were inoculated into the PK-15 cells (PCV1 free) and serially passaged 10 times.Identifications were done by PCR method and immunofluorescent assay of the tenth generation of cell cultures, and the specific band could be amplified and a strong bright green fluorescence in the nucleus of PK-15 cells could be detected.The results demonstrated that the isolated strain named as FF strain belonged to PCV2.

Key words: porcine circovirus type 2; isolation; identification; FF strain

YIN Xiu-feng,DING Mei-juan,QIN Jin-jin,ZHANG Xiao-fei. Isolation and Identification of Porcine Circovirus Type 2 FF Strain[J]. .

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Isolation and Identification of Porcine Circovirus Type 2 FF Strain

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