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Antimicrobial Resistance and Pulse Field Gel Electrophoresis Analysis of Salmonella agona Strains from Poultry in Shanghai

YANG Xiao-wei1,KUANG Dai1,PAN Hai-jian1,SU Xu-dong1,WU Li-ping2,ZHANG Jian-min1   

  1. (1.College of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;2.Shanghai Shanjin Biological Science and Technology Co.,Ltd.,Shanghai 201109,China)
  • Received:2013-08-06 Online:2014-02-20 Published:2014-03-27

Abstract: To investigate the antimicrobial resistance and molecular types of S.agona strains isolated from poultry in Shanghai from 2010 to 2011,K-B method recommended by WHO was used to detect 14 strains of S.agona from poultry.Results were analyzed according to the breakpoints of CLSI,PFGE was used for molecular typing.14 strains were sensitive to 5 kinds of antibiotics.High resistant to sulfisoxazole and high moderate sensitivity to streptomycin.14 strains of S.agona were classified to 6 PFGE patterns.Some S.agona strains isolated from 2010 to 2011 were showed highly homology.Meanwhile,the relativity between antimicrobial susceptibility and PFGE patterns was remarkable.

Key words: Salmonella agona; antimicrobial resistance; pulse field gel electrophoresis; poultry