表达猪圆环病毒2型核衣壳蛋白重组杆状病毒的构建与鉴定

Abstract

Abstract: A pair of primers was designed base on the genome of porcine circovirus type 2 (PCV2) retrieved from GenBank, which could amplify the whole coding sequence of Cap gene. The PCR product was cloned into pFastBacHTA and then sequenced. Subsequently, the positive plasmid was transformed into DH10Bac cells and the recombinant plasmid with Cap gene of PCV2 (Bacmid-Cap) was identified by PCR method. The recombinant plasmid was extracted and transfected into sf9 cells and the recombinant baculovirus expressing Cap protein of PCV2 (rBacmid-Cap) was rescued, which established the basis for the further research on subunit vaccine against PCV2.

Key words: porcine circovirus type 2; Cap gene; clone; transfect; recombinant baculovirus

CLC Number:

S855.3

SONG Qing-qing, LI Qun, LI Tian-tian, HUANG Hai-qiong, FAN Juan, LI Yu-he. Construction and Identification of Recombinant Baculovirus Expressing Cap Protein of Porcine Circovirus Type 2[J]. , 2014, 41(12): 94-98.

References

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Construction and Identification of Recombinant Baculovirus Expressing Cap Protein of Porcine Circovirus Type 2

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