冰鲜鸡生产链中沙门氏菌的分离鉴定及PFGE分型研究

›› 2014, Vol. 41 ›› Issue (12): 62-66.

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Isolation, Identification and PFGE Analysis of Salmonella Isolated from Cold Fresh Chicken Production Chain

CUI Ke-qi, LI Jing-yi, FENG Sai-xiang, HE Xian-hui, LIAO Ming, XU Cheng-gang

Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agricultural, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

Received:2014-05-11 Online:2014-12-20 Published:2014-12-25

Abstract

Abstract: To understand the distribution and spread of Salmonella isolated from cold fresh chicken production chain, we collected samples from a large-scale farm, and did the thorough research about serotype analysis, and pulsed-field gel electrophoresis (PFGE) typing analysis on the basic of identification. National standards and PCR were performed for the isolation and identification of Salmonella. The Salmonella detection rate of the kinds of farms, broiler farms, slaughterhouse and sales market were 1.46%(7/480), 7.00%(21/300), 62.86%(22/35), 54.67%(41/75), respectively. The detection rate in healthy chicks and dead embryos from hatchery were 1.11%(2/180), 12.00%(9/75), respectively. The PFGE genotyping of 102 Salmonella indicates yielded 24 PFGE types. Salmonella detection rate had increased along the production chain, slaughter and marketing might be the important part of Salmonella contamination. It was existing the phenomenon of Salmonella along the production chain horizontal spread.

Key words: Salmonella; pulsed-field gel electrophoresis; cold fresh chicken production chain

CLC Number:

R378.2⁺2

CUI Ke-qi, LI Jing-yi, FENG Sai-xiang, HE Xian-hui, LIAO Ming, XU Cheng-gang. Isolation, Identification and PFGE Analysis of Salmonella Isolated from Cold Fresh Chicken Production Chain[J]. , 2014, 41(12): 62-66.

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Isolation, Identification and PFGE Analysis of Salmonella Isolated from Cold Fresh Chicken Production Chain

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