鸡传染性贫血病病毒TaqMan实时荧光定量PCR检测方法的建立

Abstract

Abstract: A fluorescence quantitative PCR assay was developed using primers and probes designed according to a high conservative sequence of chicken infectious anemia virus (CAV). Detection of viral DNA copy number was determined by extrapolation from a CAV plasmid-based standard curve. The results showed that the estimated average viral copy number could detect 1.8×10¹ copies/μL of plasmid DNA with high reproducibility (CAV of the intra or inter) assay was below 2%. Furthermore, 1 day-old SPF chicken were inoculated with CAV and the virus loads were detected in multiple organs. The results showed that the virus could be detected in the brain, heart, liver, spleen, lung, kidney, thymus, bursa and serum and the virus loads in these organs showed no significant difference. No cross-reaction was detected with other avian virus.

Key words: chicken infectious anemia virus; TaqMan Real-time PCR; probe

CLC Number:

S852.65⁺9.2

GUO Cui-li, ZHANG Chun-hong, GUO Peng-ju, ZHANG Jian-feng, SHEN Hai-yan, PAN Jia-qiang. Development of TaqMan Fluorescence Quantitative PCR Assay for Detection of Chicken Infectious Anemia Virus[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(12): 45-49.

References

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Development of TaqMan Fluorescence Quantitative PCR Assay for Detection of Chicken Infectious Anemia Virus

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