养鸭场主要细菌分离鉴定及致病性研究

Abstract

Abstract: Bacteria were isolated from the duck organs of six duck farm in Guizhou province.According to the bacteria culture characteristics, gram stain's and microscope and biochemical properties, 137 strains bacteria of 7 genus were isolated, Escherichia, Staphylococcus and Riemerella were 3 main bacteria.Selecting main pathogenic bacteria to dilute into 10⁹ CFU/mL bacteria suspension, BALB/c mice were intraperitoneal injected 0.3 mL each, pathogenicity was analysed by statisticsing its mortality rate, observing its clinical symptoms and autopsy lesions.The results showed that the Escherichia and Staphylococcus caused the BALB/c mice to die and clearly visibled the autopsy lesions, fatality rate were 23.3% and 16.7%, respectively, while there was on pathogenicity and fatality rate was 0 when injected Riemerella, the control group was 0.The results indicated that there exists mixed infection in the 6 duckery, the Escherichia and Staphylococcus have pathogenicity to mice, however Riemerella has no pathogenicity to the mice.

Key words: duck farm; bacteria; isolation and identification; mice; pathogenicity

CLC Number:

S858.32

LI Shi-jing, JI Xin-qin, WEN Ming, FU Xin-liang, RUAN Yong, LIU Ting-jiang, CHEN Jia-qi, SHI Jing-ying. Study on Isolation and Pathogenicity Test of Main Bacteria in Duck Farm[J]. , 2014, 41(12): 267-271.

References

1 王永坤,朱国强,金山,等.水禽病诊断与防治手册[M].上海:上海科学技术出版社,2002.
2 云水丽,杨勇,王小波,等.11型鸭疫里默氏杆菌分离鉴定及致病性研究[J].中国预防兽医学报,2009,31(8):605～609.
3 许虹,杜海燕,牛艺儒,等.鸡大肠杆菌耐药质粒的研究进展[J].山东畜牧兽医,2009,37(4):22～23.
4 齐冬梅,赖月辉,巫月生,等.鸭疫里氏杆菌的分离鉴定[J].广东畜牧兽医科技,2009,34(3):39～41.
5 刘洋,王玉田,杨龙锋,等.北京某鸭场鸭疫里默氏菌与大肠杆菌混合感染的诊治[J].中国兽医杂志,2014,50(1):32～37.
6 李刚.邹平县鸭源大肠杆菌分离鉴定及耐药性检测[J].中国动物检疫,2013,30(12):66～70.
7 汪铭书,程安春,李淑梅,等.鸭疫里氏杆菌、鸭源致病性大肠埃希氏菌和鸭沙门氏菌的RAPD研究[J].中国兽医科技,2004,34(10):3～8.
8 陈伯伦.鸭病[M].北京:中国农业出版社,2008.
9 赵宝华,范建华.2株鸭疫里默氏杆菌的分离鉴定及致病性研究[J].江苏农业科学,2010(5):321～322.
10 季权安,鲍国连,韦强,等.鸭疫里氏杆菌—大肠杆菌二联四价苗研究[J].浙江农业学报,2007,19(2):111～114.
11 胡清海,刘晓文,赵世华,等.应用PCR技术检测鸭疫里默氏杆菌的研究[J].中国预防兽医学报,2002,24(6):471～473.
12 陆承平.兽医微生物学[M].第4版.北京:中国农业出版社,2007.
13 傅心亮,嵇辛勤,文明,等.贵州鸭疫里默氏杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2013,40(8):155～158.
14 蔡秀磊,韦强,崔言顺,等.浙江省22株鸭疫里氏杆菌的血清型鉴定及耐药性研究[J].浙江农业学报,2007,19(1):46～49.
15 Sandhu T S.Immunogenicity and safety of a live Pasteurella anatipestifer vaccine in White Pekin ducklings:Laboratory and field trials[J].Avian Pathology,1991,20:423～432.
16 Segers P,Mannheim W,Vancanneyt M,et al.Riemerella anatipestier gen.nov.,comb.nov.,the causative agent of septicemia anserum exsudativa,and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group[J].Int J Syst Bacteriol,1993,43(4):768～776.

[1]	YANG Qinglin, WU Hao, LIU Jiayan, FU Yuxin, ZHOU Shuaishuai, LU Han, DING Manyi, LI Yao. Study of Quercetin Feed Additives on Prolonging Storage Time of Eggs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2342-2353.
[2]	LI Xiaoyu, LI Ruoyu, WANG Xiao, WANG Lili, LI Jibin, LI Shuying, XU Yongping. Research Progress on the Influence of Filamentous Phages on Host Bacterial Adaptability [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2496-2506.
[3]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[4]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.
[5]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[6]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[7]	LIU Caixia, SHI Weidi, KOU Lijun, MA Xun, WANG Jing, GAO Shengjie, LYU Shuangfei, CHEN Xuke, ZENG Dongdong, KONG Cuilian, REN Huijie. Regulation of Transcription of Key Virulence Factors of Listeria monocytogenes by LIPI-4 EⅡC [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1340-1351.
[8]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[9]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[10]	GUO Bingyu, LI Li, LI Chen, KONG Xianghui. Role and Molecular Mechanism of Mpeg1 in Pathogen Clearance [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1584-1592.
[11]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[12]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[13]	SHI Hongzhao, LIU Yang, YAO Ruizhi, LIU Peng, LI Shize. Effects of Cold Stress Rewarming on Neurobehavioral Changes in ICR Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 932-941.
[14]	WANG Wu, WANG Bi, BAI Yu, CHEN Qirong. Research Progress on Antibacterial Activity of Modified Lysozyme [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 942-951.
[15]	CHEN Shaojun, LI Gang, NAI Zida, LIU Di, JIANG Xinpeng. Research and Application of Gene-edited Probiotics in Animal Intestinal Health [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1016-1024.

Study on Isolation and Pathogenicity Test of Main Bacteria in Duck Farm

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments