草原红牛胰岛素样生长因子结合蛋白6基因外显子2的遗传多态性及遗传效应分析

Abstract

Abstract: This experiment was conducted to study the slaughter of the relationship between meat traits and genotypes. This study selected Red cattles as the experimental group, using PCR-SSCP method of detection of bovine IGFBP6 gene exon 2 polymorphism analysis with the slaughter of the relationship between meat traits by the GLM model of the SPSS 13.0 statistical software to analysis. The results showed that IGFBP6 gene had a polymorphic site A180G in exon 2, at the same time had three genotypes of AA, AB and BB. Between the three genetypes,AB and BB genetypes were very significant higher than AA genetype in net meat weight (P<0.01),and were significant higher than AA genetype in eye muscle area and bone weigh (P<0.05), while in the ante-mortem live weight, carcass weight, kidney fat weight and rib fat weight in the three genotypes were no significant differences (P>0.05). These results indicated that the slaughter of meat was associated with certain traits, accessibility for the future protection of breeding species of Red cattle to provide a theoretical basis, and Red cattle of beef with traits improve the delivery methods and theoretical on the guidance.

Key words: Red cattle; IGFBP6 gene; genetic polymorpism; genetic effects

CLC Number:

S813.3

YANG Chun, LIU Zhen, LI Chun-yi. Genetic Polymorphisms of Exon 2 of IGFBP6 Gene and its Genetic Effects in Red Cattle[J]. , 2014, 41(11): 227-231.

References

1 刘桂兰,蒋思文,熊远著,等. IGF2基因PCR-RFLP多态性与脂肪沉积相关性状关联分析[J]. 遗传学报,2003, 30(12):1107～1112.
2 孙维斌,陈宏,雷雪芹,等. IGFBP3 基因多态性与秦川牛部分屠宰性状的相关性[J]. 遗传,2003,25(5):511～516.
3 张金玉,秦立红,张国梁,等. 草原红牛胰岛素样生长因子Ⅰ受体基因3'UTR序列多态性分析[J]. 中国畜牧兽医, 2010, 37(7):103～105.
4 李向阳,张嘉保. 草原红牛肉用性状微卫星标记研究[J]. 中国牛业科学,2006,32(1):9～13.
5 国家畜禽遗传资源委员会. 中国畜禽遗传资源志——猪志[M]. 北京:中国农业出版社,2011.
6 房希碧,贾泓瑶,张昕,等. 西藏小型猪与军牧一号白猪IGFBP-6基因表达差异分析[J]. 吉林农业大学学报,2013,35(6):727～731.
7 金海国,俞美子. 草原红牛主要经济性状的遗传参数估计[J]. 河北农业大学学报,2002, 25(3):74～77.
8 俞美子. 草原红牛部分数量性状的遗传参数及育种值的估计[J]. 延吉:延边大学,2001.
9 胡沈荣,于姣,蓝贤勇,等.西农萨能奶山羊IGFBP3基因多态与产羔数、体尺性状的关联分析[J].西北农林科技大学学报:自然科学版,2007,5(11):1～5.
10 党瑞华,魏伍川,陈宏,等. IGFBP3 基因多态性与鲁西牛和晋南牛部分屠宰性状的相关性[J]. 中国农学通报,2005,21(3):19～22.
11 郭玉姣,唐国庆,李学伟,等.猪脂肪组织中IGF2和IGFBP3基因表达的发育性变化及其品种差异[J]. 遗传,2008,30(5):602～606.
12 萨姆布鲁克 J, 弗里奇E F, 曼尼阿蒂斯T. 分子克隆实验指南[M]. 第2版. 北京:科学出版社, 1996.
13 Bach L A,Hsieh S, Brown A L, et al. Recombinant human insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-Ⅱ-induced differentiation of L6A1 myoblasts[J].Endocrinology,1994,135(5):2168～2176.
14 Bienvenu G, Seurin D, Grellier P, et al. Insulin-like growth factor bingding protein-6 transgenic mice:Postnatal growth, brain development, and reproduction abnormalities[J]. Endocrinology, 2004, 145(5):2412～2420.
15 Knoll A, Putnová L, Dvorák J, et al. A Ncil PCR-RFLP within intron 2 of the porcine insulin-like growth factor 2(IGF2) gene[J]. Animal Genetics, 2000, 31(2):150～151.
16 Kolaikov O, Putnov L, Urban T, et al. Association of the IGF2 gene with growth and meat efficiency in Large White pigs[J]. J Appl Genet, 2003, 4(44):509～513.
17 Lee H Y, Chun K H, Liu B, et al. Insulin-like growth factor bingding protein-3 inhibits the growth of non-small cell lung cancer[J]. Cancer Res, 2002, 62(12):3530～3537.
18 Moschos S J, Mantzoros C S. The role of the IGF system in cancer, from basic to clinical studies and clinical applications[J]. Oncology, 2002, 63(4):317～332.
19 Pollak M. Insulin and insulin-like growth factor signalling in neoplasia[J]. Nature Reviews Cancer, 2008, 8(12):915～928.

[1]	REN Jianming, JI Peng, ZHANG Xiaosong, WEI Yanming, ZHU Xiaopeng, ZHU Yueming. Effects of Jianpi Xiaodao Chinese Herbal Compound on Growth Performance, Rumen Fermentation Parameters and Serum Indexes of 6-month-old Simmental Crossbred Cattles [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2593-2600.
[2]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[3]	LI Yulong, LYU Shijie, ZHAI Yaying, ZHANG Zhijie, ZHU Xiaoting, ZHANG Zijing, CHEN Fuying, SHI Qiaoting, WANG Eryao. Cloning and Bioinformatics Analysis of POLB Gene in Jiaxian Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 887-896.
[4]	ZHANG Hua-ju, XU Ya-bo, WANG Lin-lin, SUN Bin-bin, LI Zhi-gang, DING Ya-jun, SUN Hong-xia. Comparative Study on the Beef Traits Between Jiaxian Red Cattle and Crossbred Beef Cattle [J]. , 2017, 44(12): 3482-3490.
[5]	YANG Hua-ming，BAN Zhi-bin，LIANG Hao，XIANG Yan，ZHANG Guo-liang，ZHAO Yu-min. Effects of Cellulose and Active Dry Yeast on Energy Metabolism of Grassland Red Cattle [J]. , 2014, 41(1): 96-101.
[6]	BAN Zhi-bin, ZHANG Guo-liang, YANG Hua-ming, XIANG Yan, ZHAO Yu-min. Effect of Active Dry Yeast and Cellulose Enzymes on the Concentration of Rumen VFA and Emission of Methane in Grassland Red Cattle [J]. , 2013, 40(2): 57-61.
[7]	ZHAO Jin-feng;QI Meng;DONG Hai-ju;LI Kui;NING Chang-shen. Survey on the Prevalence of Intestinal Parasites in Jiaxian Red Cattle，Henan Province [J]. , 2011, 38(3): 176-177.
[8]	ZHANG LI-chun;HU Cheng-hua;LI Zhao-zhi;CAO Yang;ZHOU Guo-li;JIN Hai-guo. Polymorphism Analysis on Intron 14 4685 bp Loci of CAPN1 Gene in Different Cattle Population [J]. , 2010, 37(8): 107-111.

Genetic Polymorphisms of Exon 2 of IGFBP6 Gene and its Genetic Effects in Red Cattle

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments