Abstract: This experiment was aimed to study the inhibition effects of nitric oxide (NO) on Brucella in the process of Brucella infecting mice macrophage, and the interaction relations between NO and asymmetry dimethylarginine (ADMA). Brucella vaccine strain M5 was used to infect mice macrophags, then NO production and ADMA levels were determined by the Griess reagent method and ELISA. CFU were counted for each infection period. Results suggest that the NO production of macrophages rose when they were infected by Brucella, and the extracellular release quantity was significanty higher than the intracellular (P<0.05). Compared with the control group, the differences were significant (P<0.05). But the ADMA levels showed the opposite trend with NO content. There were extremely significant differences (P<0.01) compared with the control group. Combined with CFU count results, it showed that the inhibition effects of NO on Brucella only occurred at the early stage of the infection (the former 12 h), but not at the later period (the last 12 h). The ADMA had certain inhibitory effect on NO generated in the process of Brucella infecting mice macrophage.

Key words: Brucella; mice macrophages; nitric oxide; asymmetric dimethylarginine