荷斯坦牛乳源金黄色葡萄球菌流行性及其与乳源总菌群的耐药性分析

Abstract

Abstract: The aim of the study was to investigate and compare the prevalence and antimicrobial resistance profiles of Staphylococcus aureus (S.aureus) and total coliforms isolated from cow milk at three Chinese Holstein herds in northern China region.A total of 181 milk samples including productive group,milk from tank and mastitis of three dairy farms were collected and screened for the presence of S.aureus incubated onto Baird-Parker agar.S.aureus islolates were characterized using PCR for its unique nuc gene.Agar dilution method was used for the detection of antimicrobial profiles of S.aureus and the milk samples.Of the 181 milk samples,100% were contaminated with the microorganisms,16.03% were positive for S.aureus (n=48).For the total coliforms,the resistance was most frequently observed to the beta-lactam antibiotic (oxacillin,ampicillin,cefoxitin,cefoperazone),which was above 89%;followed by erythrocin,rifampicin,tetracycline,vancomycin,trimethoprim,amoxycillin/clavulanate potassium with above 80%;chloramphenicol (above 50%);the least was amikacin (18% to 58%).The antimicrobial resistance profiles of S.aureus in three herds indicated that a high degree of resistance was observed for ciprofloxacin,cefoxitin and trimethoprim;while they were sensitive to amikacin,chloramphenicol,gatifloxacin and vancomycin.The majority of the isolates were multidrug resistance.The results laid a foundation for improving the udder health and treating the mastitis scientifically.

Key words: cow milk; Staphylococcus aureus; total coliforms; agar dilution method

CLC Number:

S852.61⁺1

AN Jing, WANG Xiao, WANG Xin, GUO Gang, WANG Ya-chun, YU Ying. Prevalence and Antimicrobial Resistance of Staphylococcus aureus and Total Coliforms Isolated from Holstein Milk[J]. , 2014, 41(10): 213-218.

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Prevalence and Antimicrobial Resistance of Staphylococcus aureus and Total Coliforms Isolated from Holstein Milk

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