亚洲黑熊主要组织相容性复合体DQA及DRB基因外显子2多态性分析

Abstract

Abstract: In this study,we analyze the allelic diversity of the DQA and DRB gene exon 2 locus on major histocompatibility complex (MHC) gene of Asiatic Black bear. Researced by cloning,sequencing and statistical analysis in the 40 Asiatic Black bear (Ursus thibetanus) from Yunnan province. The results showed that nine alleles of the DQA gene exon 2 and thirteen alleles of the DRB gene exon 2 were identified from 40 individuals. Relative to the giant panda,the diversity of DQA and DRB locus in Asian Black bears were more polymorphic. Comparisons of non-synonymous and synonymous substitutions in the antigen binding sites of deduced amino acid sequences indicated evidence for balancing selection on DRB and DQA locus.

Key words: Ursus thibetanus; DQA gene; DRB gene; polymorphic; balancing selection

CLC Number:

S813.3

GAO Wen-jie, ZHU Zi-yan. Polymorphic Analysis of MHC-DQA and MHC-DRB Gene Exon 2 in Asiatic Black Bear(Ursus thibetanus)[J]. , 2014, 41(10): 157-164.

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Polymorphic Analysis of MHC-DQA and MHC-DRB Gene Exon 2 in Asiatic Black Bear(Ursus thibetanus)

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