乌蒙凤鸡FGF23基因多态位点筛查与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.07.016

Abstract

Abstract: In order to find out the single nucleotide polymorphism (SNP) of fibroblast growth factor 23 (FGF23) gene and lay a foundation for further research on the genetic variation of FGF23 gene,SNP of FGF23 gene was screened by direct sequencing of PCR products in Wumeng Crested chickens,and the genetic characteristics,linkage disequilibrium,haplotype,diplotype and bioinformatics were analyzed.The results showed that 3 moderately polymorphic SNPs were detected in the promoter region of FGF23 gene in Wumeng Crested chickens,which were g.73424341 C>A,g.73424417 A>G and g.73424701 T>A,respectively,each SNP produced 3 genotypes.χ² test results found that only g.73424417 A>G extremely significantly deviated from the Hardy-Weinberg equilibrium (P<0.01).The results of linkage disequilibrium,haplotype and diplotype analysis showed that there was only strong linkage disequilibrium between g.73424417 A>G and g.73424701 T>A among 3 SNPs,and a total of 4 haplotypes and 8 diplotypes were found,diplotype H1H2 had the highest frequency,followed by H3H4,and the lowest frequency was H2H2.Bioinformatics analysis results found that the core promoter region of FGF23 gene was likely to be in the range of -400 to -300 bp,3 SNPs were not in the core promoter region in this study.The total number of transcription factors were 293 and 300 of g.73423055-g.73425055 region before and after mutation,respectively.The transcription factor was unchanged (all were ICSBP) of g.73424341 C>A before and after mutation.g.73424417 A>G caused the transcription factor to change from GR to C/EBPalp.There was no transcription factor of g.73424701 T>A before and after mutation.It was speculated that SNPs might have an important influence on the regulatory elements of the promoter.

Key words: Wumeng Crested chickens; FGF23 gene; polymorphic loci; bioinformatics

CLC Number:

S813.3

TIAN Qin, ZHOU Yi, ZHANG Minghua, ZHOU Bijun, CHENG Zhentao, WANG Kaigong, WEN Ming. Polymorphism Screening and Bioinformatics Analysis of FGF23 Gene in Wumeng Crested Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2113-2121.

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Polymorphism Screening and Bioinformatics Analysis of FGF23 Gene in Wumeng Crested Chickens

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