Abstract: To explore the effects of chicken transfer factor liposome on mouse macrophage phagocytosis, digestion and absorption, 30 SPF female mice with almost the same size and weight were selected and divided into 3 groups randomly and each group with 10 mice. Each group was intraperitoneal injected with CTF, CTFL and normal saline respectively every other day and 5 times with 2 mL for each one, with the normal saline group as the control. After injecting 11 and 21 d, mouse macrophage phagocytosis rate was detected. At the same time, the duodenum, jejunum and ileum of each mouse were taken out and fixed in formalin liquid, for paraffin section, HE stain, then examined under a microscope and the length of intestinal villi were carefully measured. The results showed that macrophage phagocytosis rates of groups CTF and CTFL were extremely significantly different comparing with the control group (P＜0.01), while groups CTF and CTFL had no significant difference (P＞0.05). At the 11th and 21th day of the experiment, compared with the control group, the average length of duodenum of groups CTF and CTFL were significantly different (P＜0.05), the average length of duodenum of group CTFL was longer than that of group CTF, while the average length of jejunum and ileum of all groups had no significant difference (P＞0.05).The results indicated that both CTF and CTFL could increase the immune function of the mouse and CTFL was better for promoting digestion and absorption.

Key words: transfer factor liposome; macrophage; mouse; length of intestinal villus