猪硫辛酸合成酶基因的克隆及组织表达

Abstract

Abstract: The objective of this expriment was to clone pig lipoic acid synthase (Lias) gene by using electronic extension and RT-PCR and to study its tissue distribution in pig. A pair of cloning primers was designed based on the in silico sequence information and was used to clone and analyse the gene of pig Lias (No:JN797612.1). The positive clone was identified and sequenced, at the same time the Lias gene organization distribution rule was analyzed by RT-PCR. The length of pig Lias was 1119 bp, including an ORF of 1119 bp, encoding 372 amino acids. Identity analysis showed that the Lias nucleotide sequence in pig shared 71.5%, 69.0%, 77.3%, 70.4% homology with that of Mus musculus, Rattus norvegicus, Homo sapiens, Bos taurus, respectively, the predicted peptide shared 91.5%, 79.4%, 89.4%, 88.2% homology with that of Mus musculus, Rattus norvegicus, Homo sapiens, Bos taurus, respectively. The phylogenetic tree demonstrated that pig was the closest to Mus musculus and Rattus norvegicus. Semi-quantitative RT-PCR analysis showed that Lias expressed in heart, liver, kidney, adipose tissue, spleen, skin, rectum, lung, duodena jejunum, ileum and muscle, but it was higher in the kidney and fat, and then expressed in rectun and ileum.

Key words: pig; Lias gene; clone; tissue expression

CLC Number:

Q786

CAO Jing-jing, ZHANG Xiao-mei, LU Wei-fei, GUO Yu-jie, HAN Li-qiang, WANG Lin-feng, YANG Guo-yu. Cloning and Tissue Expression of Pig Lipoic Acid Synthase Gene[J]. , 2013, 40(9): 75-79.

References

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Cloning and Tissue Expression of Pig Lipoic Acid Synthase Gene

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