利用SUMO表达系统高效表达猪O型口蹄疫病毒VP0、VP1、VP3基因

Abstract

Abstract: Structural protein precursor P1 gene of foot and mouth disease virus (FMDV) serotype O in swine was synthesised according to published sequences in GenBank.The structural protein VP0,VP1 and VP3 gene primers were designed depending on these specific sequences,and were obtained by PCR method according to the P1 gene, respectively.The PCR products were cloned into Blunt vector,and were digested and ligated into the prokaryotic expression vector SUMO.These recombinant expression plasmids,SUMO-VP0, SUMO-VP1 and SUMO-VP3,were transformed into E.coli BL21(DE3)plysS.For detecting the efficient expression,these fusion protein products expressed by the E.coli were checked by SDS-PAGE electrophoresis,and molecular weight were 55,48 and 40 ku,respectively.The largest ratio of the bacterial protein was induced under the conditions of IPTG concentration of 1.0 mmol/L,temperature of 37 ℃ and last for 5 h. The results of Western blotting showed that the fusion proteins had a good reaction with the FMDV positive serum.

Key words: FMDV serotype O in swine; VP0,VP1,VP3 gene; SUMO; high expression

CLC Number:

Q786

SONG Ni, WEN Yong-jun, WANG Feng-xue, WU Hua. Using SUMO Expression System to Efficiently Express VP0,VP1,VP3 Protein of Foot and Mouth Disease Virus Serotype O in Swine[J]. , 2013, 40(9): 61-65.

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Using SUMO Expression System to Efficiently Express VP0,VP1,VP3 Protein of Foot and Mouth Disease Virus Serotype O in Swine

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