Abstract: We extracted chicken infectious anemia virus (CIAV) genome from liver tissue,amplified and gained vp1 gene using the polymerase chain reaction (PCR).By using the recombinant DNA technology, we digested insert DNA and pET-32a (+) with restriction enzyme BamHⅠand XhoⅠ, and then purified pET32a(+) vector DNA and insert DNA, after that ligating pET32a(+) vector DNA and insert DNA. Transform recombinant pET32a-vp1 into non-expression host E.coli DH5α competent cells. Using colony PCR to identify positive clones and verify reading frame by sequencing.Transform the plasmid pET32a-vp1 into host E.coli plys. The recombinant bacteria was induced by IPTG and analyzed with SDS-PAGE. The results showed that vp1 gene was expressed in E.coli at high level. ELISA identified target protein was vp1 fusion protein. The present study would be helpful for the development of ELISA diagnostic kit with recombinant antigen of CIAV.

Key words: chicken infectious anemia virus(CIAV); vp1; prokaryotic expression