表面展示猪圆环病毒2型衣壳蛋白的重组杆状病毒的构建与鉴定

Abstract

Abstract: The Cap gene partially deleted nuclear localization signal sequence(pdCap) of porcine circovirus 2(PCV2)was cloned into pBACsurf-1 between the upstream gp64 signal sequence and downstresm gp64 mature domain to construct the recombinant plasmid pBACsurf-1-pdCap.The fusion gene containing pdCap and gp64 was subcloned into pFastBac^TM Dual to construct the recombinant plasmid pFastBac-pdCap.And then the plasmid was transformed into E.coli DH10Bac competent cells to obtain the recombinant shuttle vector Bacmid-pdCap.Eventually,the recombinant Bacmid-pdCap was transfected into Sf9 cells to produce the recombinant baculovirus BV-pdCap mediating by Lipofectamine^TM2000.Indirect immunofluorescent and Western blotting assay indicated that the recombinant baculovirus BV-pdCap could express pdCap protein with significant antigencity.

Key words: porcine circovirus 2; pdCap protein; baculovirus expression vector system

CLC Number:

Q785

JIN Li-ming, ZHANG Chun-lei, LIAO Ming, FAN Hui-ying. Construction and Identification of Recombinant Baculovirus Surface Displaying the Cap Protein of Porcine Circovirus Type 2[J]. , 2013, 40(7): 6-10.

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Construction and Identification of Recombinant Baculovirus Surface Displaying the Cap Protein of Porcine Circovirus Type 2

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