薄层层析－紫外分光光度法测定预混剂中的恩拉霉素

›› 2013, Vol. 40 ›› Issue (4): 125-128.

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Determination of Enramycin in Premix by TLC-UV Spectrophotometry

ZHOU Peng-fei¹, CHEN Min¹, FANG Zhong²

1. College of Food Science & Bioengineering,Zhejiang Gongshang University, Hangzhou 310012,China;
2. Zhejiang Chyszern technology Co.,Ltd.,Hangzhou 310014,China

Received:2012-09-20 Online:2013-04-20 Published:2013-04-19

Abstract

Abstract: A method of TLC-UV was set up for determining enramycin in premix. Target components were extracted by acetone aqueous solution. Using TLC plate, a mixture of N-Butanol∶glacial acetic acid∶water (2∶1∶1) was used as developer. Fixing the position under the light of a UV lamp(254 nm), detection wavelength was 271 nm. The absorbance and quantity of sample application showed a good linear relationship when the quantity of enramycin ranged from 20 to 166 μg (r=0.9989) with average recovery of 99.85% and RSD value of 3.1%(n=9). The content of enramycin in premix detected by this method was almost the same as that was detected by HPLC. This method which was simple, rapid and accurate,large amount of analysis,could be used to the quantitative analysis of enramycin in premix.

Key words: enramycin; TLC-UV spectrophotometry; premix 

CLC Number:

S859.79⁺6

ZHOU Peng-fei, CHEN Min, FANG Zhong. Determination of Enramycin in Premix by TLC-UV Spectrophotometry[J]. , 2013, 40(4): 125-128.

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Determination of Enramycin in Premix by TLC-UV Spectrophotometry

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